A therapeutic concentrate of coagulation factors II, IX and X from citrated, factor VIII-depleted plasma.

A simple procedure is described for t he large-scale absorption on t o DEAEcelluiose of coagulation factors II , I X and X from citrated, factor VHI-depleted plasma. The coagulation factors are elutcd frontally f rom the exchanger in a high yield and hi a form suitable f o r therapeutic use, without further fractionation. The lyophilised con­ centrate is very stable without the addition of heparin and, when redlssoh'cd to isoosraolar solution, contains approximately 30 T.'/ml factors II, I X a a d X, 250-300 times purified f rom the starting plasma. The effectiveness of the concentrate in the treatment of haemophilia B is discussed.